@article{120666,
  author = {X. H. Kang and J. Wang and H. Wu and I.A. Aksay and J. Liu and Y. H. Lin},
  title = {Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing},
  abstract = { Direct electrochemistry of a glucose oxidase (GOD)-graphene-chitosan nanocomposite was studied. The immobilized enzyme retains its bioactivity, exhibits a surface confined, reversible two-proton and two-electron transfer reaction, and has good stability, activity and a fast heterogeneous electron transfer rate with the rate constant (k(s)) of 2.83 s(-1). A much higher enzyme loading (1.12 x 10(-9) mol/cm(2)) is obtained as compared to the bare glass carbon surface. This GOD-graphene-chitosan nanocomposite film can be used for sensitive detection of glucose. The biosensor exhibits a wider linearity range from 0.08 mM to 12 mM glucose with a detection limit of 0.02 mM and much higher sensitivity (37.93 mu A mM(-1) cm(-2)) as compared with other nanostructured supports. The excellent performance of the biosensor is attributed to large surface-to-volume ratio and high conductivity of graphene, and good biocompatibility of chitosan, which enhances the enzyme absorption and promotes direct electron transfer between redox enzymes and the surface of electrodes. },
  year = {2009},
  journal = {Biosensors \& Bioelectronics},
  volume = {25},
  pages = {901-905},
  month = {12/2009},
  isbn = {0956-5663},
  language = {eng},
}